In Vitro Propagation of Avocado (Persea americana Mill.) Abstract
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چکیده
A method with high success frequency for propagation of avocado is described. Embryonic axes of mature avocado seeds from six Floridian cultivars were excised and cultured in Murashige and Skoog (MS) medium containing growth regulators. Two types of cytokinins were used: benzyladenine (BA) and thidiazuron (TDZ). Explants were incubated in the dark for 7-10 days to reduce browning, then transferred to 18 h photoperiod. Multiple shoots were subcultured for further multiplication. Shoots were rooted in MS medium with 2 mg/1 indolebutyric acid (IBA). This procedure could have application in improving the cloning of avocado varieties and rootstocks having desirable characteritics.
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